국립부경대학교 생물공학과

2004 - 2011 부경대학교 생물공학과 학사

2011 - 2013 부경대학교 생물공학과 석사

2013 - 2016 부경대학교 생물공학과 박사

2021 - 현재 부경대학교 생물공학과 조교수

2017 - 2021 한국생명공학연구원 박사후 연구원

미생물 유전공학 실험실에서는 인간 생활사에 영향을 미치는 개별 미생물 또는 이들의 모임인 마이크로바이옴에 대해 연구하고있습니다.

생명의 기본 분자인 미생물 유래 DNA, RNA, protein, metabolite을 분석하여 우리인간에 어떻게 이롭게 적용할지 가설을 세우고 증명합니다.

NGS, Bioinformatics, CRISPR 등의 최신 기술을 이용해 기초과학적 연구에서부터 산업적 적용에 이르는 미생물 활용기술을 개발하여 관련분야 인적자원 공급과 해양미생물 분야 세계최고 수준의 연구 결과 도출을 목표로 합니다.

미생물학, 응용미생물학, 미생물유전공학, 산업미생물학, 기초생물공학실험Ⅰ

• Jang, W. J., Lee, G. H., Lee, J. M., Kim, T. Y., Jeon, M. H., Kim, Y. H., & Lee, E. W. (2021). Improving enzyme activity, thermostability and storage stability of β-1, 3-1, 4-glucanase with poly-γ-glutamic acid produced by Bacillus sp. SJ-10. Enzyme and Microbial Technology, 143, 109703.
• Kang, M. K., Kim, M. H., Park, H. R., Liu, M. J., Jin, C. Z., Park, S. H., ... & Kim, C. J. (2020). Nematicidal Activity of Teleocidin B4 Isolated from Streptomyces sp. against Pine Wood Nematode, Bursaphelenchus xylophilus. Pest Management Science.
• Yang, Y., Jin, C. Z., Jin, F. J., Li, T., Lee, J. M., Kim, C. J., ... & Jin, L. (2020). Caulobacter soli sp. nov., isolated from soil sampled at Jiri Mountain, Republic of Korea. International Journal of Systematic and Evolutionary Microbiology, 70(7), 4158-4164.
• Lee, J. M., Jang, W. J., Lee, E. W., & Kong, I. S. (2020). β-glucooligosaccharides derived from barley β-glucan promote growth of lactic acid bacteria and enhance nisin Z secretion by Lactococcus lactis. Lwt, 122, 109014.
• Lee, J. M., Jang, W. J., Park, S. H., & Kong, I. S. (2020). Antioxidant and gastrointestinal cytoprotective effect of edible polypeptide poly-γ-glutamic acid. International journal of biological macromolecules, 153, 616-624.
• Siddique, M. P., Jang, W. J., Lee, J. M., Hasan, M. T., Kim, C. H., & Kong, I. S. (2019). Detection of Vibrio anguillarum and Vibrio alginolyticus by singleplex and duplex loop-mediated isothermal amplification (LAMP) assays targeted to groEL and fklB genes. International Microbiology, 22(4), 501-509.
• Tak, J. Y., Jang, W. J., Lee, J. M., Suraiya, S., & Kong, I. S. (2019). Expression in Lactococcus lactis of a β-1, 3-1, 4-glucanase gene from Bacillus sp. SJ-10 isolated from fermented fish. Protein expression and purification, 162, 18-23.
• Jang, W. J., Choi, S. Y., Lee, J. M., Lee, G. H., Hasan, M. T., & Kong, I. S. (2019). Viability of Lactobacillus plantarum encapsulated with poly-γ-glutamic acid produced by Bacillus sp. SJ-10 during freeze-drying and in an in vitro gastrointestinal model. LWT, 112, 108222.
• Jang, W. J., Lee, J. M., Hasan, M. T., Lee, B. J., Lim, S. G., & Kong, I. S. (2019). Effects of probiotic supplementation of a plant-based protein diet on intestinal microbial diversity, digestive enzyme activity, intestinal structure, and immunity in olive flounder (Paralichthys olivaceus). Fish & shellfish immunology, 92, 719-727.
• Kim, J. H., Lee, J. M., Jang, W. J., Park, H. D., Kim, Y. O., Kim, C. H., & Kong, I. S. (2019). Efficient production of poly γ­d­glutamic acid from the bloom­forming green macroalgae, Ulva sp., by Bacillus sp. SJ­10. Biotechnology and bioengineering, 116(7), 1594-1603.
• Jang, W. J., Lee, J. M., Hasan, M. T., & Kong, I. S. (2019). Fusion of the N-terminal domain of Pseudomonas sp. phytase with Bacillus sp. phytase and its effects on optimal temperature and catalytic efficiency. Enzyme and microbial technology, 126, 69-76.
• Hasan, M. T., Je Jang, W., Lee, J. M., Lee, B. J., Hur, S. W., Gu Lim, S., ... & Kong, I. S. (2019). Effects of immunostimulants, prebiotics, probiotics, synbiotics, and potentially immunoreactive feed additives on olive flounder (Paralichthys olivaceus): a review. Reviews in Fisheries Science & Aquaculture, 27(4), 417-437.
• Lee, J. M., Jang, W. J., Hasan, M. T., Lee, B. J., Kim, K. W., Lim, S. G., ... & Kong, I. S. (2019). Characterization of a Bacillus sp. isolated from fermented food and its synbiotic effect with barley β-glucan as a biocontrol agent in the aquaculture industry. Applied microbiology and biotechnology, 103(3), 1429-1439.
• Jang, W. J., Lee, J. M., Park, H. D., Choi, Y. B., & Kong, I. S. (2018). N-terminal domain of the beta-propeller phytase of Pseudomonas sp. FB15 plays a role for retention of low-temperature activity and catalytic efficiency. Enzyme and microbial technology, 117, 84-90.
• Jang, W. J., Lee, J. M., Kim, Y. R., Hasan, M. T., & Kong, I. S. (2018). Complete genome sequence of Bacillus sp. SJ-10 (KCCM 90078) producing 400-kDa poly-γ-glutamic acid. Current microbiology, 75(10), 1378-1383.
• Lee, J. M., Kim, J. H., Kim, K. W., Lee, B. J., Kim, D. G., Kim, Y. O., ... & Kong, I. S. (2018). Physicochemical properties, production, and biological functionality of poly-γ-D-glutamic acid with constant molecular weight from halotolerant Bacillus sp. SJ-10. International journal of biological macromolecules, 108, 598-607.
• Cho, H. J., Jang, W. J., Moon, S. Y., Lee, J. M., Kim, J. H., Han, H. S., ... & Kong, I. S. (2018). Immobilization of β-1, 3-1, 4-glucanase from Bacillus sp. on porous silica for production of β-glucooligosaccharides. Enzyme and microbial technology, 110, 30-37.
• Suraiya, S., Lee, J. M., Cho, H. J., Jang, W. J., Kim, D. G., Kim, Y. O., & Kong, I. S. (2018). Monascus spp. fermented brown seaweeds extracts enhance bio-functional activities. Food bioscience, 21, 90-99.
• Suraiya, S., Siddique, M. P., Lee, J. M., Kim, E. Y., Kim, J. M., & Kong, I. S. (2018). Enhancement and characterization of natural pigments produced by Monascus spp. using Saccharina japonica as fermentation substrate. Journal of Applied Phycology, 30(1), 729-742.
• Siddique, M. P., Jang, W. J., Lee, J. M., Ahn, S. H., Suraiya, S., Kim, C. H., & Kong, I. S. (2017). gro EL is a suitable genetic marker for detecting Vibrio parahaemolyticus by loop­mediated isothermal amplification assay. Letters in applied microbiology, 65(2), 106-113.
• Kang, D. S., Moon, S. Y., Cho, H. J., Lee, J. M., & Kong, I. S. (2017). Interaction of a 22 kDa Peptidyl Prolyl cis/trans Isomerase with the Heat Shock Protein DnaK in Vibrio anguillarum. Journal of microbiology and biotechnology, 27(3), 644-647.
• Lee, J. M., Moon, S. Y., Kim, Y. R., Kim, K. W., Lee, B. J., & Kong, I. S. (2017). Improvement of thermostability and halostability of β-1, 3-1, 4-glucanase by substituting hydrophobic residue for Lys48. International journal of biological macromolecules, 94, 594-602.
• Park, Y., Moniruzzaman, M., Lee, S., Hong, J., Won, S., Lee, J. M., ... & Bai, S. C. (2016). Comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, Platichthys stellatus. Fish & shellfish immunology, 59, 351-357.
• Rafiquzzaman, S. M., Ahmad, M. U., Lee, J. M., Kim, E. Y., Kim, Y. O., Kim, D. G., & Kong, I. S. (2016). Phytochemical Composition and Antioxidant Activity of Edible Red Alga H ypnea musciformis from B angladesh. Journal of Food Processing and Preservation, 40(5), 1074-1083.
• Oh, R., Moon, S. Y., Cho, H. J., Jang, W. J., Kim, J. H., Lee, J. M., & Kong, I. S. (2016). Cloning and Characterization of Phosphomannomutase/Phosphoglucomutase (pmm/pgm) Gene of Vibrio anguillarum Related to Synthesis of LPS. Microbiology and Biotechnology Letters, 44(3), 355-362.
• Rafiquzzaman, S. M., Ahmed, R., Lee, J. M., Noh, G., Jo, G. A., & Kong, I. S. (2016). Improved methods for isolation of carrageenan from Hypnea musciformis and its antioxidant activity. Journal of applied phycology, 28(2), 1265-1274.
• Ahmed, R., Rafiquzaman, S. M., Hossain, M. T., Lee, J. M., & Kong, I. S. (2016). Species-specific detection of Vibrio alginolyticus in shellfish and shrimp by real-time PCR using the groEL gene. Aquaculture international, 24(1), 157-170.
• Rafiquzzaman, S. M., Kim, E. Y., Lee, J. M., Mohibbullah, M., Alam, M. B., Moon, I. S., ... & Kong, I. S. (2015). Anti-Alzheimers and anti-inflammatory activities of a glycoprotein purified from the edible brown alga Undaria pinnatifida. Food Research International, 77, 118-124.
• Lee, J. M., Kim, Y. R., Kim, J. K., Jeong, G. T., Ha, J. C., & Kong, I. S. (2015). Characterization of salt-tolerant β-glucosidase with increased thermostability under high salinity conditions from Bacillus sp. SJ-10 isolated from jeotgal, a traditional Korean fermented seafood. Bioprocess and biosystems engineering, 38(7), 1335-1346.
• Jo, G. A., Lee, J. M., No, G., Kang, D. S., Kim, S. H., Ahn, S. H., & Kong, I. S. (2015). Isolation and characterization of a 17-kDa FKBP-type peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum. Protein expression and purification, 110, 130-137.
• Kim, E. Y., Rafiquzzaman, S. M., Lee, J. M., Noh, G., Jo, G. A., Lee, J. H., & Kong, I. S. (2015). Structural features of glycoprotein purified from Saccharina japonica and its effects on the selected probiotic properties of Lactobacillus plantarum in Caco-2 cell. Journal of Applied Phycology, 27(2), 965-973.
• Rafiquzzaman, S. M., Min Lee, J., Ahmed, R., Lee, J. H., Kim, J. M., & Kong, I. S. (2015). Characterisation of the hypoglycaemic activity of glycoprotein purified from the edible brown seaweed, U ndaria pinnatifida. International Journal of Food Science & Technology, 50(1), 143-150.
• Kim, S. H., Lee, J. M., Kang, D. S., Kim, D. G., Ahn, S. H., & Kong, I. S. (2014). Expression, purification and characterization of soluble recombinant peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum. Protein expression and purification, 101, 54-60.
• Kim, Y. R., Kim, E. Y., Lee, J. M., Kim, J. K., & Kong, I. S. (2013). Characterisation of a novel Bacillus sp. SJ-10 β-1, 3？1, 4-glucanase isolated from jeotgal, a traditional Korean fermented fish. Bioprocess and biosystems engineering, 36(6), 721-727.
• Hossain, M. T., Kim, Y. R., Kim, E. Y., Lee, J. M., & Kong, I. S. (2013). Detection of Vibrio cholerae and Vibrio vulnificus by duplex PCR specific to the groEL gene. Fisheries science, 79(2), 335-340.
• Kim, Y. R., Kim, E. Y., Choi, S. Y., Hossain, M. T., Oh, R. K., Heo, W. S., ... & Kong, I. S. (2012). Effect of a probiotic strain, Enterococcus faecium, on the immune responses of olive flounder (Paralichthys olivaceus). Journal of microbiology and biotechnology, 22(4), 526-529.

 

미생물 유전공학 연구실 (Microbial genetic engineering Lab)
홈페이지 https://jmlee84.wixsite.com/mgel / https://biotech.pknu.ac.kr/view.do?no=528